DNA damage induced p53 stabilization: no indication for an involvement of p53 phosphorylation
نویسندگان
چکیده
منابع مشابه
DNA Damage-Induced Phosphorylation of p53 Alleviates Inhibition by MDM2
DNA-damaging agents signal to p53 through as yet unidentified posttranscriptional mechanisms. Here we show that phosphorylation of human p53 at serine 15 occurs after DNA damage and that this leads to reduced interaction of p53 with its negative regulator, the oncoprotein MDM2, in vivo and in vitro. Furthermore, using purified DNA-dependent protein kinase (DNA-PK), we demonstrate that phosphory...
متن کاملPhosphorylation of Ser-20 mediates stabilization of human p53 in response to DNA damage.
Stabilization of p53 in response to DNA damage is caused by its dissociation from Mdm2, a protein that targets p53 for degradation in the proteasome. Dissociation of p53 from Mdm2 could be caused by DNA damage-induced p53 posttranslational modifications. The ATM and ATR kinases, whose activation in response to ionizing radiation (IR) and UV light, respectively, is required for p53 stabilization...
متن کاملMulti-site phosphorylation of p53 by protein kinases inducible by p53 and DNA damage.
Introduction The p53 tumour suppressor protein is a key element in the cell’s defence against tumour development, and as such is the focus of considerable study for the development of anticancer therapies (for recent reviews see [1,2]). p53 is a potent transcription factor, which is activated in response to signals arising from DNA damage, viral infection, cytokines, hypoxia, activated oncogene...
متن کاملPhosphorylation of Daxx by ATM Contributes to DNA Damage-Induced p53 Activation
p53 plays a central role in tumor suppression. It does so by inducing anti-proliferative processes as a response to various tumor-promoting stresses. p53 is regulated by the ubiquitin ligase Mdm2. The optimal function of Mdm2 requires Daxx, which stabilizes Mdm2 through the deubiquitinase Hausp/USP7 and also directly promotes Mdm2's ubiquitin ligase activity towards p53. The Daxx-Mdm2 interacti...
متن کاملEnhanced DNA damage-induced p53 peptide phosphorylation and cell-cycle arrest in Sjögren's syndrome cells.
BACKGROUND Cells from primary Sjögren's syndrome (SS) patients have been reported to show alterations in DNA repair and p53 expression. The DNA-dependent protein kinase (DNA-PK) autoantigen may be involved in both of these alterations in relation to cellular DNA damage responses. We conducted this study of cell-cycle kinetics and p53 to find additional evidence for an abnormal stress response r...
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ژورنال
عنوان ژورنال: Oncogene
سال: 1999
ISSN: 0950-9232,1476-5594
DOI: 10.1038/sj.onc.1202480